You can follow the above reaction in a test tube using a dye that turns purple in the presence of pyruvate (more purple, the more pyruvate that is made). You discover an inhibitor of terpase and want to determine if it is a competitive or non-competitive inhibitor. You set up the following reactions and measure the amount of purple made using a spectrophotometer. The results are below:

Is this a COMPETETIVE or NON-COMPETITIVE inhibitor (circle one)?

Transcribed Image Text:

ABCD Abs= 0.63 0.02 UU 0.56 0.21 A = Enzyme + Substrate B=Inhibitor added C = Inhibitor + 5x Substrate D = Inhibitor + 10x Substrate ABCD Abs= 0.63 0.02 UU 0.56 0.21 A = Enzyme + Substrate B=Inhibitor added C = Inhibitor + 5x Substrate D = Inhibitor + 10x Substrate

Answer rating: 100% (QA)

Competitive inhibition A competitive inhibitor is the one that competes with substrate for ... View the full answer