Question: ( 1 0 ) The PUC 1 8 vector is 2 . 7 kb and has a multiple cloning site with H - B -

(10) The PUC 18 vector is 2.7 kb and has a multiple cloning site with H-B-E sites in the order listed. A 4 kb region of capybara DNA is cloned into the Bam HI site of pUC18 and digested with the following restriction enzymes and the resulting fragment sizes in kilobases (kb) are shown below. Please draw the restriction map of the recombinant plasmid. Show your work if you need to decide of two possible sites. Indicate sizes for B&E double diges.
BamHI: 4.0,2.7
HindIII: 6.2,0.5
EcoRI: 3.7,3.0
H&E: 3.5,2.7,0.3,0.2
B&E:
H&B: 3.5,2.7,0.5
( 1 0 ) The PUC 1 8 vector is 2 . 7 kb and has a

Step by Step Solution

There are 3 Steps involved in it

1 Expert Approved Answer
Step: 1 Unlock blur-text-image
Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock
Step: 3 Unlock

Students Have Also Explored These Related Biology Questions!