1)

1) a) Create a restriction enzyme that will remove the gene of interest (indicated by the highlighted region below) and create sticky ends. You must show the prime ends on the restriction enzyme you make. Explain why it would be a good choice. (2) 3' ATT GCA GGA TCC AAA TTT AGC GAT ATT GGA TCC GCA TGC AAT 5' 5' TAA CGT CCT AGG TTT AAA TCG CTA TAA CCT AGG CGT ACG TTA 3' b) Show the resulting fragments of the digest below: (1)2) Given the following DNA sequence, draw the gel pattern that will result from Sanger dideoxy sequencing. Remember to label your gel clearly. (you can draw on a separate piece of paper and upload your drawing in the space below) (3) 5' ATGGCTAGACTATGC 3'