Question: 1 . 2 2 Using Moment Analysis to Evaluate Chromatography Column Performance On 2 different days 2 weeks apart for a 5 0 c m

1.22 Using Moment Analysis to Evaluate Chromatography Column Performance On 2
different days 2 weeks apart for a 50cm long ion exchange chromatography column, a
step change in sodium chloride concentration is introduced at the column inlet in order
to evaluate the column performance. Conductivity is measured at the column outlet as a
function of the effluent volume, as shown in Table P7.22.
TABLE P7.22
Determine the non-Gaussian HETP for each case. How do these cases compare
in terms of column efficiency? If there is a significant difference in column effi-
ciency between the two cases, what may be a reason for the discrepancy?
 1.22 Using Moment Analysis to Evaluate Chromatography Column Performance On 2

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