1. (2 points) Construct a standard curve using Excel, following the guidelines given in the protocol. Print the graph and attach it to this page with a staple or paper clip. Physically mark your graph (by hand or using Excel) to show the approximate positions of the unknown data points. 2. (1 point) Write the regression equation for the standard curve here. 3. (1 point) Give the unknown BSA concentration calculated from each of the unknown assay tubes. 4. (1 point) Averaging the results obtained from the unknown assay tubes, what is the concentration of BSA in the unknown sample? What is the code number (or letter) on the label of your unknown sample? 5. (1 point) Due to a student's poor pipetting technique, all the solutions pipetted actually contained 10% more volume than the recipe calls for. If this student performed the same lab exercise you did, would she or he get the same value for the concentration of your unknown BSA solution? Explain. 6. (1 point) You would like to determine the concentration of purified protein. Using a Bradford Assay, you generate the following standard curve. Calculate the concentration of your protein (show your calculations and report your answer to three significant digits). Note: The Abs595 of your protein that was diluted by a factor of 10 is 1.25. 7. (1 point) Attach your notebook page with your data to your report.

Reference for question 4: code number 6