$16$ Multiple Choice $1$ point

Cells were genetically altered to express only a mutant form of Ran GTPase. This Ran mutant can no longer bind to its GEF protein, Rcc$1.$ How would you predict this mutant Ran protein will localize in cells?

Over time, many new proteins with nuclear localization signals will be synthesized. Where will the vast majority of these newly$-$synthesized NLS proteins be found?

Mutant Ran will be found only in the cytoplasm, and the NLS proteins will be found in the nucleus.

Mutant Ran will be found only in the nucleus, and the NLS proteins will be found in the cytoplasm.

Mutant Ran and the NLS proteins will both be found in the nucleus.

With no GTP in the cell, mRNA cannot be transcribed, and none of these proteins will be expressed in the first place.

Mutant Ran and the NLS proteins will both be found in the cytoplasm.

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