1. A 1.00 L Tris buffer solution is made by mixing 121.14 g of Tris base (MW = 121.14 g/mol), 84 mL of 10 M HCl and 916 mL of water. What is the pH of the prepared Tris buffer?

2. A lab mate accidentally spilled 10 mL of 10 M HCl onto the petri dish culturing Azotobacter. How much do you expect the pH of the solution to change (little, a lot or not at all?)

3. Calculate the resulting pH after the HCl spill.