1. A genomic library of a prokaryotic. organism is often con- structed by cloning the products...

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1. A genomic library of a prokaryotic. organism is often con- structed by cloning the products of a Sau3Al partial digest of the genomic DNA into a BamHI site of the vector. a. Why are two different enzymes used in this experi- ment? b. What is a partial digestion, and how is it performed? c. Why is partial digestion often used for constructing genomic libraries? 2. Describe the principal features of the pUC cloning system 1. A genomic library of a prokaryotic. organism is often con- structed by cloning the products of a Sau3Al partial digest of the genomic DNA into a BamHI site of the vector. a. Why are two different enzymes used in this experi- ment? b. What is a partial digestion, and how is it performed? c. Why is partial digestion often used for constructing genomic libraries? 2. Describe the principal features of the pUC cloning system

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1 a Sau3AI is the restriction enzyme in genomic DNA and BamHI is the vector DNA restriction enzyme u... View the full answer