1. Describe how to make 1000 ml of 70% ethyl alcohol using a v:v dilution with...

 

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1. Describe how to make 1000 ml of 70% ethyl alcohol using a v:v dilution with 95% ethyl alcohol and deionized water. Show your work. (1 point) 2. How much agarose powder (g) would you use to make a 50 ml, 1.4% agarose gel? (1 point) 3. You have run a gel with 5 uL of amplified DNA in addition to 1 uL of 6X loading dye. Comparing the brightness of this band to your marker, you estimated that your amplified DNA is approximately 62 ng. What was the concentration of your amplified DNA that was loaded? You do not need to account for the volume of loading dye in your calculations. (1 point) 1. Describe how to make 1000 ml of 70% ethyl alcohol using a v:v dilution with 95% ethyl alcohol and deionized water. Show your work. (1 point) 2. How much agarose powder (g) would you use to make a 50 ml, 1.4% agarose gel? (1 point) 3. You have run a gel with 5 uL of amplified DNA in addition to 1 uL of 6X loading dye. Comparing the brightness of this band to your marker, you estimated that your amplified DNA is approximately 62 ng. What was the concentration of your amplified DNA that was loaded? You do not need to account for the volume of loading dye in your calculations. (1 point)

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1 For 70 ethanol we need 70mL of 100 ethanol for 100mL solution If stock ethanol is 95 then we n... View the full answer