Question: 1. During a fed-batch bioreactor run, you are adjusting pH using a 1 Molar solution of Ca(OH)2 (~1500 mOsm). Feed solution with osmolarity of 1400

1. During a fed-batch bioreactor run, you are adjusting pH using a 1 Molar solution of Ca(OH)2 (~1500 mOsm). Feed solution with osmolarity of 1400 mOsm is used for fed-batch operation. The starting cell culture medium has an osmolarity of 300 mOsm. The total base addition was 40 mL/L of culture and the total feed was 350 mL/L. Assume that:

1) In given conditions the base dissolves completely. Hint: remember the molarity of dissolved compound vs undissolved, # of particles is crucial. 2) The total Volume in the Bioreactor is 1 L.

a) estimate the final osmolarity in the bioreactor. b) estimate the final osmolarity if each feed (on top of existing amount) added 50 mls of 1 Molar solution of glucose. (Here use initial setting without the base; any glucose that is already in the feed or base medium is accounted for).

Step by Step Solution

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock blur-text-image

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Students Have Also Explored These Related Chemical Engineering Questions!

Refer to Section 2.4.9 given in the course text on pages 31-33. This example refers to the results given in Figure 2.14 that were produced by solving the model eqs. 2-98 to 2-101 using a numerical...

Biological reactions that involve microorganisms and enzyme catalysts are pervasive and play a crucial role in the natural world. Without such bioreactions, plant and animal life as we know it simply...

A - false 1 . 2 . 1 . Which of the following statements are correct for the production of human insulin in E . colit? Insulin is a complex protein undergoing glycosylation. E . coll is chosen as host...

Suppose that the fed-batch bioreactor in figure is converted to a continuous, stirred-tank bioreactor (also called a chemostat) by adding an exit stream. Assume that the inlet and exit streams have...

with all the details please A fed-batch bioreactor is used for penicillin production. The flow rate to the bioreactor was 50L/h, the initial volume and the initial cell concentration were 500L and...

Develop the dynamic model for each process in group: 1. Stirred tank heating process (Variable holdup) 2. Electrically heated Stirred tank 3. Steam heated Stirred tank 4. Liquid Storage system 5....

Question 1 45 Marks A group of chemical and biochemical engineers is considering the design of a control system to control the biomass production from a bioreactor. Figure 1 provides the schematic...

Analyze the following industrial processes from process control viewpoint by identifying the (i) control objective, (ii) control, manipulated, and disturbance variables, (iii) operating...

Q2) A feed substrate is fed into an anaerobic fed-batch bioreactor at a rate of 0.5t(m3/h), with t has the following characteristics: YXs=0.20g/g KS=0.12kg/m3 Kmax=0.12.31h1 ms=0.12kg/kgh Si=50kg/m3...

Bioreactions are often carried out in batch reactors. The fed-batch bioreactor model in Section 2.4.9 is also applicable to batch reactors if the feed flow rate F is set equal to zero. Using the...

You work for a small accounting firm in Halifax and are filling in as a temp during the winter semester. It is February 5th 2020 and you are working on the January month-end accounting file for a...

Why is it important for the accounting department of a company to receive data from sales and shipping? What internal control function is served by this process?

6. Fill-In Questions The only trading bloc that has eliminated all its internal tariffs is________________ . (LO6)

Gaining control of someone else's computer to carry out illicit activities without the owner's knowledge is known as spyware. electronic eavesdropping on computer monitors. hijacking. phreaking....

4. The majority of farm subsidies flow toward . LO22.4 a. Poor, small-scale farmers. b. Rich, large-scale farmers. c. Government employees. d. Grain wholesalers.

2. Use supply and demand curves to depict equilibrium price and output in a competitive market for some farm product. Then show how an above-equilibrium price floor (price support) would cause a...

1. Suppose that the demand for olive oil is highly inelastic. Also suppose that the supply of olive oil is fixed for the year. If the demand for olive oil suddenly increases because of a shortage of...