1.

Eukaryotic gene expression is different from prokaryoticexpression in which of the following ways?

	A. Eukaryotes have operons.
	B. Prokaryotic DNA associates with histones.
	C. Eukaryotic DNA forms chromatin.
	D. Prokaryotes export mRNA from the nucleus.
	E. None of the above.

2.

Changes of chromatin structure that support activation oftranscription in eukaryotes include:

	A. sliding of nucleosomes, making DNA accessible.
	B. opening of chromatin.
	C. activating DNase I.
	D. A and B.
	E. All of the above.

3.

The "histone code can include all of the following EXCEPT:

	A. acetylation to activate transcription.
	B. acetylation to repress transcription.
	C. methylation to activate transcription.
	D. methylation to repress transcription.
	E. Actually, all of these are included.

4.

DNA methylation leads to methyl groups being added to CpG,which in turn:

	A. recruits acetylases, which lead to chromatinaccessibility.
	B. recruits acetylases, which lead to chromatininaccessibility.
	C. recruits deacetylases, which lead to chromatinaccessibility.
	D. recruits deacetylases, which lead to chromatininaccessibility.
	E. None of the above.

5.

DNA sequences that are involved in the regulation oftranscription in eukaryotes include all of the followingEXCEPT:

	A. the core promoter.
	B. the regulatory promoter.
	C. the operator.
	D. enhancers.
	E. Actually, all of these are included.

6.

Transcriptional activators are proteins that:

	A. bind DNA in a sequence-specific fashion.
	B. associate with other proteins to increasetranscription.
	C. are regulated by coactivators.
	D. A and B.
	E. All of the above.

7.

Transcriptional activators can bind:

	A. regulatory promoters.
	B. coactivators.
	C. enhancers.
	D. A and B.
	E. All of the above.

8.

The control of transcriptional activation by GAL4 resemblesprokaryotic systems in that:

	A. GAL4 only binds DNA when it binds its ligand,galactose.
	B. GAL4 comes off DNA when it binds galactose.
	C. GAL4 activation is blocked by another protein in the absenceof galactose.
	D. GAL4 activation is blocked by another protein in thepresence of galactose.
	E. None of the above.

9.

Repressors function by:

	A. binding to the regulatory promoter.
	B. binding to silencers.
	C. blocking RNA polymerase binding.
	D. A and B.
	E. All of the above.

10.

Enhancers, unlike regulatory promoters,

	A. do not have to be in a specific location.
	B. do not have to be in a specific orientation.
	C. are often transcribed.
	D. A and B.
	E. All of the above.

11.

Since enhancers function independently of orientation andposition, their effect is regulated by:

	A. insulators.
	B. silencers.
	C. mediators.
	D. All of the above.
	E. None of the above.

12.

In addition to being regulated at the transcriptional level,eukaryotic genes can also be regulated by all of the followingEXCEPT:

	A. alternative splicing.
	B. control of capping.
	C. control of degradation.
	D. Actually, all of these are levels of control.
	E. Actually, none of these are eukaryotic controlmechanisms.

13.

RNA interference can repress proteins by all of the followingmechanisms EXCEPT:

	A. blocking translation.
	B. promoting mRNA degradation.
	C. promoting histone acetylation.
	D. promoting histone methylation.
	E. Actually, interference can take any of these forms.

14.

Regulation of translation in eukaryotes has been shown to takethe form of:

	A. attenuation.
	B. regulation of initiation factors.
	C. regulation of elongation factors.
	D. regulation of the initiator tRNA.
	E. None of the above.