Question: 1) For each cuvette (each different concentration of PNPP) plot absorbance vs. time, with time as the independent variable. The initial slope for each cuvette

1) For each cuvette (each different concentration of PNPP) plot absorbance vs. time, with time as the independent variable. The initial slope for each cuvette will be the initial rate for that initial concentration of PNPP.

2) Using the data from the first step of the analysis, plot initial rate vs. initial concentration of PNPP with initial concentration of PNPP as the independent variable.

3) Using the data from the second step of the analysis, create a Lineweaver-Burke plot. Using this plot determine the KM and Vmax for the enzyme. Be sure to include units for these values.

1) For each cuvette (each different concentration of PNPP) plot absorbance vs.

time, with time as the independent variable. The initial slope for each

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