Question: 1. The output profile from a chromatographic column is given in figure 1 . The conditions were as follows: Column: 0.4625cm column packed with 10m

1. The output profile from a chromatographic column is given in figure 1 . The conditions were as follows: Column: 0.4625cm column packed with 10m reversed phase particles. h=v2+1+v312+(1+k0)2(1+k)2(k0+k+k0k)2v32+30k0(1+k0)2(1+k)2(k0+k+k0k)2v+(1+k0)(1+k)2dp2kd2kDmv reduced plate height h=dpH H=HETP=LL2= dispersion per unit column length v=Dmdpue= reduced velocity =0.7= structural parameter =2.5= structural parameter =2= structural parameter =151= structural parameter =2= tortuosity parameter Dm=2x105cm2/sec= molecular diffusivity k0=ei(1e)=0.8 a) Calculate the contributions hdisp,he,diff,hi,diff to the theoretical plate height. b) Calculate hkin. c) Calculate the desorption rate constant, kd, from the above data. d) Discuss the relative contributions to the theoretical plate height
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