[10] 4. The radius of gyration for a circle is just the radius of the circle...

 

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[10] 4. The radius of gyration for a circle is just the radius of the circle R, while the radius of gyration for a rod of length Lis L/√12. Suppose that a DNA molecule could exist either as rigid rod 100 nm long, or as a circle with this circumference. Assuming that λ = 632 nm, calculate the ratio of light scattering at 0= 90° for the rod form to that of the circle form. If the minimum signal difference that can be measured by the detector is 5%, can the breaking of the circles be detected using light scattering? Suggest another method to measure the conformation of the DNA chain. [10] 4. The radius of gyration for a circle is just the radius of the circle R, while the radius of gyration for a rod of length Lis L/√12. Suppose that a DNA molecule could exist either as rigid rod 100 nm long, or as a circle with this circumference. Assuming that λ = 632 nm, calculate the ratio of light scattering at 0= 90° for the rod form to that of the circle form. If the minimum signal difference that can be measured by the detector is 5%, can the breaking of the circles be detected using light scattering? Suggest another method to measure the conformation of the DNA chain.

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