1.15.12. . . 1...3. . 1. . 4 . . .1. . . 5 . . . 1 . . . 6 . . 1 . . . 7 . . . 1.Fill the following Matrix using the Needleman/Wunsch "Global" Dynamic Programming Sequence Alignment Method. Use Match = 1, Mismatch = 0, Gap Penalty (w) = 0. See Lecture 4 Slides as well as review the Supplement provided if having difficulty. Answer = 2 TAGCCGAT 0 0 0 0 0 0 0 0 0 A G 0 G 0 A TO 2. Perform Traceback for the Matrix filled in for Question 1 and provide the alignment as your answer. Remember, start at the lower right See Lecture 4 Slides as well as review the Supplement provided if having difficulty. Answer = ? 3. What is the Score for the Alignment in Question 2? See Lecture 4 Slides as well as review the Supplement provided if having difficulty. Answer ? 4.Generate the Local DP " Smith-Waterman" pairwise sequence alignment between the Aqp4 rat [Rattus norvegicus] Beffeq protein sequence (NP_001135838.1) and the AQP4 cow [Bos taurus] BetSeq protein sequence (NP_001304721.1). Use the "EMBOSS" tool to generate the alignment. Then copy/paste the sequence alignment returned from tool as your answer. Be sure to review Lecture 4 Slides if you are having difficulty. EMBOSS tool url: https://www.ebi.ac.uk/Tools/psalemboss_water/ Answer ? 5. What is the Score for the Alignment in Question 4? Be sure to review Lecture 4 Slides if you are having difficulty. Answer = ? O DELL