13. Watch your units carefully in the following problem and give units in all of your...

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13. Watch your units carefully in the following problem and give units in all of your answers. (There are 3 parts to this question with a total of 12 points.) Total enzyme The following data was obtained for 0.005 mM hexokinase using ATP as a substrate: [S], mM - Vo, (micromole/literYsec to maari 0.003 0.004 28 40 Change 0.010 0.020 - 70 - 95 - 112 0.040 0.100 2.000 10.000 - 140 - 128 - 139 imax a.) By inspection of the data, estimate the approximate values of Km, Vmax, and kcat for the system. (6 points) b.) Give an estimate of the catalytic efficiency of the enzyme by determining the specificity constant and comment how close it is to catalytic perfection. (4 points) c.) What assumption made in Michaelis-Menten kinetics is not true for the conditions described here? (2 points) 13. Watch your units carefully in the following problem and give units in all of your answers. (There are 3 parts to this question with a total of 12 points.) Total enzyme The following data was obtained for 0.005 mM hexokinase using ATP as a substrate: [S], mM - Vo, (micromole/literYsec to maari 0.003 0.004 28 40 Change 0.010 0.020 - 70 - 95 - 112 0.040 0.100 2.000 10.000 - 140 - 128 - 139 imax a.) By inspection of the data, estimate the approximate values of Km, Vmax, and kcat for the system. (6 points) b.) Give an estimate of the catalytic efficiency of the enzyme by determining the specificity constant and comment how close it is to catalytic perfection. (4 points) c.) What assumption made in Michaelis-Menten kinetics is not true for the conditions described here? (2 points)

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Keat EtS ES Es eney me Sy substrate ES s eney meSubstrate co... View the full answer