Question: 13. What does the Master Mix contain? 14. What are primers? Why is a primer added? 15. Once the primers bind, what occurs next?
13. What does the Master Mix contain? 14. What are primers? Why is a primer added? 15. Once the primers bind, what occurs next? 16. What does "highly conserved" mean? 17. Why are highly conserved regions important in this lab? 18. What does "highly variable" mean? 19. Why are highly variable regions important in this lab? 20. What is missing in the negative control tube? 21. What is present in the positive control tube that is not in the negative control tube? Now run the PCR. Be sure to watch the virtual lab animation before proceeding to the questions. 22. List each step of a PCR cycle, the temperature, and the duration (time). a. b.
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