19. (4 pts.) How would you make a solution that contains 5106 copies per 5L of a 4.7 kbp plasmid? You are provided a plasmid prep to use which has an A260 of 0.56 (Assume that 1bp has a mass of 660g/mol ). 20. (3 pts.) Why does an ultracentrifuge need a high vacuum and what are the components of the vacuum system in an ultracentrifuge? 21. (4 pts.) Name 4 factors that affect DNA hybridization stringency. a) b) c) d) 22. (20 pts.) You need to separate a solution of the following " G " molecules in buffer: Glycine (small zwitterionic amino acid, M.W. 75) Glucose (small nonionic monosaccharide, M.W. 180) Glycogen (large, nonionic polysaccharide, M.W. > 1,000,000) GTP (guanosine triphosphate) (small anionic nucleotide triphosphate, M.W. 523) GAPDH (protein, M.W. 37kDa, isoelectric point (pl) of pH8.5 ) What chromatographic procedures would you use to separate large amounts (milligram quantities) of these compounds from each other? Try to use the fewest steps possible