1.Find the ortholog of PP_3662 in E. coli K12 and post the fasta sequence. Align the two...
Question:
1.Find the ortholog of PP_3662 in E. coli K12and post the fasta sequence. Align the two proteins using two different programs with one showing a dot plot and insert both images in the text box.
2.The PP_3662 protein and its E. coli homolog harbor a complex domain organization. How many domains? Give their DUF or COG names and show a picture.
3.Find the recent publication (published since 2019 included) characterizing the catalytic region's substrate binding site of the E. coli homolog. Provide the PMID.
4.Complete a multiple alignment for a subset of protein sequences that are orthologs of PP_3662 (including the E. coli one) with homologs that are diverse enough to show the conserved catalytic motif identified in the preceding paper, "PGGxGTxxE". Insert the image of the alignment in the text box.
5.Generate a logo of these active site residues. In which domain are these residues located? Post the picture in the box.
Income Tax Fundamentals 2013
ISBN: 9781285586618
31st Edition
Authors: Gerald E. Whittenburg, Martha Altus Buller, Steven L Gill