Question: 1.Using the values shown in the table, calculate the pI of the dipeptide, Ser-Ile. If no value is given, assume that the group does not

1.Using the values shown in the table, calculate the pI of the dipeptide, Ser-Ile. If no value is given, assume that the group does not ionize.2.

GROUP a pK C-terminus COOH 3.5 Asp 3.9 \begin{tabular}{c} Asp \\ \hline Glu \\ \hline His \end{tabular} 4.1 6.0 \begin{tabular}{lll} \hline Cys & CH2SH & 8.4 \\ \hline N-terminus & NH3+ & 9.0 \\ \hline Tyr & CH2 & 10.5 \\ \hline Lys & CH2CH2CH2CH2NH3+ & 10.5 \\ \hline \end{tabular} Arg 12.5 Determining the sequence of the 284-residue protein bacteriorhodopsin was quite an undertaking when it was accomplished in the 1970s. The protein was cleaved into shorter peptides using cyanogen bromide; then each peptide was sequenced by cleaving it into smaller fragments. Determine the sequence of one of these peptides using the data in the table below

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