1.Why do plasmid vectors contain a polylinker?

a)To find a restriction enzyme site that is useful for inserting the fragment of

interest

b)Multiple cut sites are required for the DNA fragment to be inserted.

c)So that the plasmid can be cut into many small fragments to be inserted in the

region of interest

d) To allow the plasmid to survive on antibiotic media

2.What does blue color mean in blue-white screening with pUC18?

a)The DNA fragment was not successfullyinserted into the plasmid vector

b)The DNA fragment was successfullyinserted into the plasmid vector

c)The bacteria was successfully transformedwith the plasmidvector

d)The bacteria was not successfullytransformed with the plasmid vector

3.cDNAis synthesized from what single stranded molecule?___________

4.A plasmidvector would be a good choice for inserting a 100kbDNA fragment into a bacterium.(T or F)

5.How does gel electrophoresis separate DNA fragments on an agarosegel?

a)Longer fragments move more quickly towards the positively charged end of the gel

b)Longer fragments move more quickly towards the negatively charged end of the gel

c)Shorter DNA fragments move more quickly towards the positively charged end of the gel

d)Shorter fragments move more quickly towards the negatively charged end of the gel

6.Which of the following are true regarding Southern Blotting?

a)It can only be used with genomicDNA

b)It is used to detect specific RNA fragments

c)Only the probes which hybridize with a complementary sequence will be seen on the autoradiogram

d)The first step is to separate the nucleic acid with electrophoresis

e)The DNA run on the gel is double stranded,but the DNA that is detected by the probe is single stranded

f)Electrophoresis pulls the DNA from the gel into the membrane

7.What enzyme is used to create cDNA?

a)Reverse transcriptase

b)DNA ligase

c)Primase

d)EcoRIendonuclease

8.Recombinant DNA technology is only useful for genes linked on the same chromosome.(T or F)

9.DNA fragments are heated to 94 degrees C so that they can hybridize.(T or F)

10.Ethidiumbromide is used to visualize DNA bands on an agarosegel (T or F)

11.Two peanut cultivarsare crossed and their f1 progeny are selfed to obtain an F2 generation. The first variety (represented in well B) has a hairy stem. The second variety (represented in well C) has a glabrous(hairless) stem. This trait is controlled by a single gene, and hairy stems are completely dominant over hairless stems.The DNA from both parents and the progeny is isolated, and a portion of the gene controlling this trait is amplified using PCR and then digested withEcoR1 to reveal a restriction length polymorphism. The DNA is then run on the gel shown below. Well A is a DNA ladder. Well B is the hairy parent, well C is the hairless parent, and well D is one of the F2 progeny. What is the phenotype of the F2 plant whose genotype is shown in well D?

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