2. (15) Enzyme kinetics multiple substrates. Certain enzymes mediate catalysis of multiple substrates. Assume that a single enzyme E catalyzes the conversion of two different substrates S, and S2, to two different products P, and P2, as follows: ki k2 E + Si ESi = E + P1 (Eq. 1) k-1 k3 - E + S2 k4 ES2 = E + P2 (Eq. 2) ( k-3 (a) (11) Assume that the initial enzyme concentration [E.] is known and that the quasi-steady state assumption holds true for [ES,] and [ES2]. Derive the expression for the product formation rate v for P1, in terms of rate constants, [E.], [S], and [S2]. You must show the steps of the derivation, not just the final answer. (b) (4) Based on what you found in part (a), decide if the following statements are true or false: a. The maximum production rate of P, will not change by having two substrates. b. By having the second substrate S2, the apparent Michaelis-Menten constant Km, app for Vi increases