2. Consider again a column from problem 1). Assume that the column length is fixed at 25 cm and that you can not change the stationary phase inside the column. You are in the lab performing the experiment and you want to improve the performance of the column. What specific, practical experimental measures could you take to: a) (5 points) achieve peak separation in a shorter time? Hint: which thermodynamic factor needs to be changed here? How? 1 An LC curam of a component misluba on 25 cpacked Columns shown below. The towale was 0.46 m.in. The small peak in the beginning of the chromatogram is for the us-retained species, thus indicates the dead time for the column. The 0.0 min. print coresponds to the of the sample notion 16 12 M 10 To a) 4 points each What are the remontime species and ? How much time each component ipende in the mobile phone? w och time and component penes in the stationary phone? Determine del recept c) Calculate cloetisily factor of A with respect to Determine the full width of cach peak at the bone Calculate the rest of two posle Calculate the everage member of plures for the coule Calculate the average plate bright for the colum 2. Consider again a column from problem 1). Assume that the column length is fixed at 25 cm and that you can not change the stationary phase inside the column. You are in the lab performing the experiment and you want to improve the performance of the column. What specific, practical experimental measures could you take to: a) (5 points) achieve peak separation in a shorter time? Hint: which thermodynamic factor needs to be changed here? How? 1 An LC curam of a component misluba on 25 cpacked Columns shown below. The towale was 0.46 m.in. The small peak in the beginning of the chromatogram is for the us-retained species, thus indicates the dead time for the column. The 0.0 min. print coresponds to the of the sample notion 16 12 M 10 To a) 4 points each What are the remontime species and ? How much time each component ipende in the mobile phone? w och time and component penes in the stationary phone? Determine del recept c) Calculate cloetisily factor of A with respect to Determine the full width of cach peak at the bone Calculate the rest of two posle Calculate the everage member of plures for the coule Calculate the average plate bright for the colum