Question: 2. Substrate-Inhibited Kinetics ( 30 points): Data on enzymatic degradation of dichlorophenol at different concentrations are given in the following table: a. Determine with Lineweaver-Burke

dichlorophenol at different concentrations are given in the following table: a. Determine

2. Substrate-Inhibited Kinetics ( 30 points): Data on enzymatic degradation of dichlorophenol at different concentrations are given in the following table: a. Determine with Lineweaver-Burke plotting the constants Vmax,Km, and K2 from the equation given from the slides in Lecture 7 and in Lecture 7d in Blackboard for substrate-inhibited kinetics: Rate=(KM+CS+K2(CS)2)VMAXCS b. Determine the inhibition constant K2 from the following equation: SMAX=KM1/K2 (Problem 2 Hint: You will need to identify the parts of the data associated with high concentration of substrate and the parts of the data associated with low concentration of substrate and plot those separately to solve the problem here.)

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