2. You are presented with the following data from a series of purification steps for the enzyme comatose. Fill in the Blanks Below (2.5 points) SHOW WORK (i.e., show numbers 7800*1.125 = XXXXX) Table 1. Purification of Comatase from E. Coli Yield Volume (ml) Total Protein Total Activity Specific Activity (10/mg) Purification Factor/ (%) (mg) (10) Enrichment (level) 1. Homogenization 10,000 13,173 10,000 100 1 II. Dialysis 3,500 11,564 8,111 0.70 81.1 III. Organic 110 7,232 0.98 Extraction 10 5,500 6,800 68.0 IV. Ion exchange Chromatography V. PAGE 2 981 5,494 5.6 a. 3. Given the purification schemed data shown in Table 1, which purification step gave the greatest: (1 points) Relative increase in product purity (the greatest increase of enrichment from previous step)? b. Absolute Loss of product (greatest magnitude loss in Total activity)? Absolute reduction of product volume (i.e., the greatest decrease in actual volume- largest magnitude)? d. Relative reduction of product volume (the greatest % decrease from the previous step)? C