Question: 24.7 You are interested in purifying a sample containing alcohol dehydrogenase from horse liver. However, the sample also contains the protein catalase. These two proteins

 24.7 You are interested in purifying a sample containing alcohol dehydrogenase

24.7 You are interested in purifying a sample containing alcohol dehydrogenase from horse liver. However, the sample also contains the protein catalase. These two proteins have the following transport properties: S(s) 11.3x10-13 4.88x10-13 D (m-/s) 4.1x10-11 6.5x10-11 V (cm'/g) 0.715 0.751 Catalase Alcohol dehydrogenase Determine the molecular weight of catalase and alcohol dehydrogenase b. You have access to a centrifuge that can provide angular velocities up to 35,000 rpm. For the specieis you expect to travel the greatest distance in the centrifuge tube, determine the time it would take to centrifuge until a 3 cm displacement of the boundary layer occurs relative to the initial 5 cm location of the boundary layer relative to the centrifuge axis. c. To separate the proteins, you need a separation of at least 1.5 cm between the boundary layers associated with each protein. Using your answer in part b, will it be possible to separate the proteins by centrifugation

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