Question: 3) Consider making a chromatographic column using a stainless steel tube that is 15 cm long and has an inside diameter of 4.6 mm. The

3) Consider making a chromatographic column using a stainless steel tube

3) Consider making a chromatographic column using a stainless steel tube that is 15 cm long and has an inside diameter of 4.6 mm. The tube is packed with silicon fused core spheres that have a diameter of 5 um. The outer porous region of the spheres is 0.6 um thick and coated with C18 to act as the stationary phase. a) Assuming the particles pack the column with an efficiency 74%, how many spheres will fill the tube? b) Calculate the volume of the stationary phase in the column. You may assume this is the same volume as the porous layer of the spheres. c) Calculate the volume of the mobile phase in the column. d) Assume the mobile phase is pumped through the column at 0.75 mL/min. Calculate the mobile phase time, tm

Step by Step Solution

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock blur-text-image

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Students Have Also Explored These Related Chemical Engineering Questions!

For each of the following cases, determine an appropriate characteristic length L c and the corresponding Biot number Bi that is associated with the transient thermal response of the solid object....

Question 1 Your group is tasked with performing both an order of magnitude (Class 5) and study estimate (Class 4) for the capital cost of a styrene production plant. A process flow diagram (PFD) and...

The production of methyl ethyl ketone (MEK) is described in Appendix F, problem F.3. A preliminary design has been made for a plant to produce 10,000 metric tons per year. The major equipment items...

For example, when a ball is thrown into the air, it gains kinetic energy as it moves upward. As it reaches the top of its trajectory, this kinetic energy is converted into potential energy. When the...

amount of energy in the system remains constant throughout this process Do the entire questions: One of the most fundamental principles of physics is the conservation of energy. This principle states...

energy is converted back into kinetic energy. The total amount of energy in the system remains constant throughout this process Do the entire questions: One of the most fundamental principles of...

are the same regardless of where an observer is located. This principle has been confirmed by numerous experiments, including the famous Michelson-Morley experiment, and is a cornerstone of modern...

lativity is another key concept in physics. This principle states that the laws of physics are the same for all observers in uniform motion relative to one another. This means that there is no...

ergy in the system remains constant throughout this process Do the entire questions: One of the most fundamental principles of physics is the conservation of energy. This principle states that energy...

This means that there is no preferred frame of reference in the universe, and the laws of physics are the same regardless of where an observer is located. This principle has been confirmed by...

Suppose X, Y have joint density Find the expected value of X2 + y3, i.e find E(X2 + Y3). otherwise.

AI / CT 1 1 - 7 5 ( LO 1 1 - 2 ) Assume you asked your favorite AI learning tool "Would a taxpayer classify a car used for business as a capital asset for tax purposes?" and the AI tool responded as...

CIT 1 6 1 - Exam 1 RubricComponentYes or NoMinor Flaws 1 . Use RadioButton controls to ask the user to select a spoken language for the tour, such as English, German, or Chinese. ( - 5 ) 2 . Use...

=+3. What are the ethical implications of murky, complex writing in a document

=+2. Why is it essential to understand the writers intent before suggesting or making changes to another persons document? [LO-1]

=+4. Why is it risky to rely on spell checkers and other software tools for writing and revising? [LO-4]