3. Measuring the width, w, at the baseline on the chromatogram to calculate the resolution between the following pairs of compounds:

Trans-chlordane and cis-chlordane

Trans-nonachlor and 4,4-DDE

2,4-DDD and dieldrin

4. Measuring w_1/2 on the chromatogram, find the number of plates, N₁ and N₂, and the plate height for 2,4-DDD and dieldrin.

5. Using the number of plates N=(N₁N₂) with your values from (3), calculate what the resolution should be and compare with the measured resolution in (4) for 2,4-DDD and dieldrin.

A standard of mixed pesticides was analyzed using the GC method below:

The hold-up (void) time for this analysis was 1.43 min.

Carrier Gas: Helium, Column Flow: 1.40 ml/min, Column Lenght: 30m, Average Velocity: 34.89 cm/sec, Inner Diameter: 0.25mm, Holdup time: 1.43 min, film Thickness: 0.25m, Inlet pressure: 18.97 psi, Initial Oven Temp: 100C, Hold time 0min, Ramp Rate (C/min): 20C/min, Oven Temp: 310

Peak Number	Compound	Retention Time
1	Pentachlorobenzene	5.53
2	-BHC	6.67
3	Hexachlorobenzene	6.80
4	-BHC	7.27
5	Heptachlor	7.63
6	Metolachlor	7.84
7	Aldrin	7.98
8	Heptachlor epoxide	8.41
9	2,4'-DDE	8.59
10	trans-Chlordane	8.78
11	cis-Chlordane	8.82
12	trans-Nonachlor	8.88
13	4,4'-DDE	8.97
14	2,4'-DDD	9.06
15	Dieldrin	9.11
16	Endrin	9.29
17	2,4'-DDT	9.34
18	4,4'-DDD	9.45
19	cis-Nonachlor	9.47
20	4,4'-DDT	9.71