3: Wave bioreactor - Lecture 23, slides 26-33 (20 points) To avoid the large capital expense for a conventional pilot cell culture manufacturing facility, your biotech start-up is using single-use disposable bag bioreactors (the "Wave" bag) to produce a monoclonal antibody for clinical trials. The bag has liquid working volume of 200L. Mass transfer coefficient (kLa) using rocking agitation in the wave bag bioreactor is 3.5/hr. The specific oxygen uptake rate of your cell line is 2.710-13 mol/cellhr. The cells require that dissolved oxygen partial pressure be maintained at not less than 0.04 atm. The solubility of oxygen in cell culture medium is 0.9310-3 mol/Latm. a) If oxygen is the limiting nutrient, what is the maximum viable cell concentration that can be supported in such a bioreactor using pure oxygen swept through the bag headspace? b) Your excellent cell line produces antibody at a specific rate of 100pg/cellday in a pseudo-stationary phase. What is the maximum achievable total daily production rate of product in the 200L bag in the pseudo-stationary phase (g/day)? c) Neglecting the antibody produced during exponential growth, what titer would be achieved if viable cells are maintained at the maximum density for an 8 day pseudo-stationary phase? d) What would be the approximate cost of the consumables (200 bioreactor bag with probes, bags without probes for inoculum buildup, and culture medium at $10/L) for each 200L run