Question: (30 pts) Two sets of initial rate data were obtained for an enzyme-catalyzed reaction: So (mmol/L) vo (mmol/L-min) This enzymatic reaction obeys Michaelis-Menten kinetics,

(30 pts) Two sets of initial rate data were obtained for an

(30 pts) Two sets of initial rate data were obtained for an enzyme-catalyzed reaction: So (mmol/L) vo (mmol/L-min) This enzymatic reaction obeys Michaelis-Menten kinetics, where Vmax [So] KM + [SO] 2.0 139 3.0 179 4.0 213 Vo 10.0 15.0 313 370 Find the Michaelis-Menten constant, K, and the maximum rate of reaction, Vmax for the enzymatic reaction by using linear least squares regression method. Keep four digits after the decimal point and round-off in every step of your calculations. (Hint: Apply a suitable transformation to Michaelis- Menten equation)

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