3/29/23, 5:28 PM Quiz: Problem Set 4 Total protein activity Specific Activity Efficiency (%) Step units/mg) Overall Yield b (%) Purity (%) (mg) (units) Step Yield a (%) Crude 20,000 4,000,000 N/A homogenate 100 100 Ammonium sulfate 5,000 3,000,000 precipitation Acid 4,000 1,000,000 precipitation on-exchange 300 800,000 chromatography |00 Affinity 50 50,000 chromatography Gel filtration 45 650,000 chromatography 100 6750 00 a The step yield is calculated for each two consecutive steps. The first step is arbitrarily set to 100%. b The overall yield is calculated for each step relative to the original one. The first step is arbitrarily set to 100%. . From the information provided in the table, fill in the empty fields by calculating the specific activity (units/mg protein) of the enzyme solution, step yield, overall yield, purity, and efficiency after each purification step. Consult slide 1.11 from Module 8 Lesson 2 to help you with this problem. Express your answers in full integers. In other words, if your answer contains decimal places, round up or down the number to the nearest full integer. 2. Some purification steps are more efficient than others. Which of the purification procedures used for this enzyme is most effective? . If you were not able to figure out how to calculate the efficiency of a step, think about the step which had a high yield and a high purity. Those will be the ones that are most efficient. 3. Which of the purification procedures is least effective? 4. Compare the results of the ammonium sulfate precipitation and the acid precipitation steps. Given that these two steps utilize the same purification method and are therefore really similar, which one would you get rid of? (answer either "acid" or "ammonium sulfate") 5. Observe the data for the last step (gel filtration chromatography): given that the specific activity decreases slightly, and that the purity and yield do not offer much improvement compared to the previous step, do you recommend getting rid of it? (answer "yes" or "no") Question 18 1 pts The entropic contribution to folding is generally unfavorable since it involves transitions from large numbers of unstructured conformations to a single ordered structure. True O False