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4. (20 points) In this experiment from Ohnishi, et al., total cell lysates (TCL) prepared from embryonic fibroblasts from...

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4. (20 points) In this experiment from Ohnishi, et al., total cell lysates (TCL) prepared from embryonic fibroblasts from wild type mice or transgenic mice expressing cagA were subjected to immunoprecipitation (IP) and Western blotting (IB) using the antibodies listed. Describe how the experiment was performed, the controls used and the results obtained. C B6 CAG-cagAHs IP: IgG HA IgG HA IB: anti-HA IB: anti-phospho- tyrosine IB: anti-SHP-2 Immunoprecipitates TCL CAG-cagAHS

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Molecular Cell Biology

Molecular Cell Biology

ISBN: 978-1429234139

7th edition

Authors: Harvey Lodish, Arnold Berk, Chris A. Kaiser, Monty Krieger, Anthony Bretscher, Hidde Ploegh, Angelika Amon, Matthew P. Scott

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Question Details
Chapter # 8
Section: Exercise Questions
Problem: 14
Posted Date: September 13, 2023 13:21:56
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