Question: 4) Two unknowns (A and B) were spotted on the same TLC plate. Under UV the TLC plate is as shown below: (10 pts) a)

4) Two unknowns (A and B) were spotted on the same TLC plate. Under UV the TLC plate is as shown below: (10 pts) a) With the use of a ruler, determine the Rr value of the spot for Unknown B, following the procedure described. Show your calculations and watch your significant figures. b) What does the Rr value of Unknown B tell you about the affinity of Unknown B to the mobile phase compared to the stationary phase? Be specific. A B c) Why does A have two spots and B have only one? What conclusions can you draw about the polarity of unknowns A and B? Be specific and explain. 5) Based on the Rf values of the five analgesics we studied in the experiment, which analgesic is the most polar? Explain your rationale. (5 points)

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