Question: 4. When setting up the gel apparatus, it is necessary to make sure that the gel is positioned so that the gel (and the wells
4. When setting up the gel apparatus, it is necessary to make sure that the gel is positioned so that the gel (and the wells of the gel at the top) are facing inwards towards the upper buffer chamber. Why is this necessary? (Hint: The following site does a good job of describing SDS-PAGE setup: https://brianmccaulev.net/bio6b/6b-lab/electrophoresis/sds-page-method) Overview of Gol cot.un Depiction of the two plates that "sandwich" the qel When the gel is electrophoresed, the dye should migrate from the top of the gel to the bottom. The proteins should also do the same (although are not visible yet). What charges do you think the electrode near the top and the electrode near the bottom of the gel are when there is a curfent? (If unsure, think about the chemicals discussed in question 1)
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