45 Multiple Choice 2 points The reaction catalyzed by citrate synthase: Is an example of ordered sequential binding Involves the binding of acetyl CoA that creates a conformational change that allows for the binding of oxaloacetate Is driven by the production of a highly stable intermediate Is unfavorable and thus relies on a relatively high concentration of products to reactants to proceed as written Requires an ATP cofactor 46 Multiple Choice 2 points In sequence-dependent transcription termination, which of the following is coded into the RNA after the hairpin loop to facilitate dissociation of the RNA from the template? ACCA-sequence A string of uracil residues A region that exhibits dyad symmetry The poly-A tail O A palindromic sequence 47 Multiple Choice 2 points Eukaryotic DNA replication and DNA transcription share which of the following characteristics? O The manner in which each nucleotide is added to the strand, resulting in direct 5' to 3' synthesis Approximately the same amount of single-stranded DNA exposed at any one time The speed at which they occur The nucleotide substrates that are required for synthesis O The degree of proofreading activity that occurs 48 Multiple Choice 2 points In pyrimidine synthesis, the cleavage of pyrophosphate from PRPP provides the energy for: Addition of the R-amino group of glutamine Pyrimidine ring closure Attachment of the pyrimidine ring to the ribose sugar Inclusion of the aspartate backbone in its entirety O Conversion of UMP to CTP49 Multiple Choice 2 points The base existing "above" the sugar in a nucleic acid occurs because O Placing the base above the ring ensures that it will not be degraded when the 2'-hydroxyl group experiences nucleophilic attack The base in built above the ring in purines whereas an inversion of configuration occurs when the pyrimidine is attached to ribose Both the pyrimidine and purine bases are built above the ring O Existing above the ring allows for the hydrophobic bases to become buried in the interior of the double helix O The base is built above the ring in pyrimidines whereas the an inversion of configuration occurs when the purine is attached to ribose 50 Multiple Choice 2 points The primary driving force for ATP synthesis is: O The use of GTP and CTP as activators The spontaneous flow of protons across the outer mitochondrial membrane The spontaneous nature of the reactions involved in ATP synthesis O The charge imbalance that builds across the inner mitochondrial membrane O The free energy that comes from the proton gradient