5 6 7 8 9 10 11 12 PBS buffer PBS buffer NGF NGF NGE IGF-1 IGF-1 IGF-1 3% 2% 3% 7% 4% 220% 380% 340% 3% 0% 745% 333% 530% 3% 8% 4% 9. Can you draw the same conclusions you did in #8? Why or why not? 10. What function does the IGF-1R control serve? Now imagine if the Trka control was missing: Akt activation IGF-1R activation CO Table 4 Plate 1 2 3 4 5 6 7 8 9 10 11 12 Treatment Nothing Nothing Nothing PBS buffer PBS buffer PBS buffer NGF NGF NGF IGF-1 IGF-1 IGF-1 1% 3% 2% 3% 7% 4% 220% 380% 340% (-) 0% 2 1% 4% 4% 5% 500% 625% 400% 11. What conclusions can you draw now? Are you as confident in these conclusions as you were in Table 2? Could there be another reason, other than NGF does not cause activation of Akt, for the results shown in Table 4? 5 6 7 8 9 10 11 12 PBS buffer PBS buffer NGF NGF NGE IGF-1 IGF-1 IGF-1 3% 2% 3% 7% 4% 220% 380% 340% 3% 0% 745% 333% 530% 3% 8% 4% 9. Can you draw the same conclusions you did in #8? Why or why not? 10. What function does the IGF-1R control serve? Now imagine if the Trka control was missing: Akt activation IGF-1R activation CO Table 4 Plate 1 2 3 4 5 6 7 8 9 10 11 12 Treatment Nothing Nothing Nothing PBS buffer PBS buffer PBS buffer NGF NGF NGF IGF-1 IGF-1 IGF-1 1% 3% 2% 3% 7% 4% 220% 380% 340% (-) 0% 2 1% 4% 4% 5% 500% 625% 400% 11. What conclusions can you draw now? Are you as confident in these conclusions as you were in Table 2? Could there be another reason, other than NGF does not cause activation of Akt, for the results shown in Table 4