5. A 10.0 cm long HPLC column was attached to a 20.0 yiL loop injector and the eluate monitored at its max of 254 nm. The following data were obtained on test to for the separation was 1.00 min. the retention time for a purine based drug was 4.40 min. the half width of this peak was 0.22 min. 10.0 L injection gave a peak area of 25000 (a) Calculate the number of theoretical plates in the column. (b) Calculate the capacity retention factor (K) for the drug. (c) Calculate the change in peak area if 5.00 iL is injected (d) Calculate the change in peak area if 50.0 L is injected. (e) How would the peak area change if the detection wavelength changed to 350 nm