Question: 5) The Acetate Buffer system is commonly used in biochemistry labs, and it consists of acetic acid, and its conjugate base, usually in the form

 5) The Acetate Buffer system is commonly used in biochemistry labs,

5) The Acetate Buffer system is commonly used in biochemistry labs, and it consists of acetic acid, and its conjugate base, usually in the form of sodium acetate. A common practice for researchers is to prepare "stock solutions" of the acid and conjugate base, so that they can mix them (in smaller batches) in the correct ratios to make different solutions of varying pH. You are in the lab, and you want to make 100mL of 0.1M Acetate Buffer at pH=5.0. You have two stock solutions, one is a 0.1M solution of acetic acid, and the other is a 0.1 M solution of sodium acetate. The pKa of acetic acid is 4.75. How will you mix your stock solutions to make your desired buffer solution? Considering that the pKa of acetic acid is 4.75, what do you think is the useful buffering range for Acetate Buffer

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