Question: Describe a sample preparation combined with direct high performance liquid chromatography (HPLC) method for the separation and determination of the analytes (suggest column packing,

Describe a sample preparation combined with direct high performance liquid chromatography (HPLC)

Describe a sample preparation combined with direct high performance liquid chromatography (HPLC) method for the separation and determination of the analytes (suggest column packing, mobile phase, solubility of analytes in mobile phase and detector that would be the most suitable). Provide justification for your answers. CH, CH3 H2N' H,N SMZ SDZ pKa = 7.4 pK, = 6.5 H2N SMX pKe = 5.7 ZI

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