Question: 6. Create a simple outline (yes, you can use bullets and outlines!) starting from plasmid DNA with your clone in it (pick one, anyone),

6. Create a simple outline (yes, you can use bullets and outlines!)

6. Create a simple outline (yes, you can use bullets and outlines!) starting from plasmid DNA with your clone in it (pick one, anyone), transform competent cells (choose the cells, describe competent cells and transformation process), and how you would go all the way through from lysis to purification of a GST-Tagged protein.

Step by Step Solution

★★★★★

3.45 Rating (165 Votes )

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock blur-text-image

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Students Have Also Explored These Related Chemistry Questions!

One way that bacterial cells regulate DNA replication is by GATC methylation sites within the origin of replication. Would this mechanism work if the DNA was conservatively (rather than...

Outline all steps in a synthesis that would transform 2-propanol (isopropyl alcohol) into each of the following: (a) (b) (c) (d) (e) CI

You are studying a circular plasmid DNA molecule of size 10.5 kilobase pairs (kb). When you digest this plasmid with restriction endonucleases BamHI, EcoRI, and HindIII, singly and in all possible...

Create your page(s) using s Kung Fu Panda Po Sequence as the page title(s) (tag). Save the first page as index.htm (or index.php). Use a HTML form to capture user input. Submit the form to a second...

Scenario 1 (Mr Barney). Relates to questions 3 - 14 Mr Allen Barney is an 82-year-old man of Aboriginal and Torres Strait Islander decent, living in Queensland. He has been diagnosed with Alzheimer's...

Assignment 1 1701ICT Creative Coding Griffith College This assignment should be submitted in two milestones: Milestone 1: Questions 1, 2, 3, 4 (due end of Week 4) Milestone 2: Questions 5, 6, 7 (due...

Decision Tree Assignment What is a Decision Tree? A decision tree is a visual tool that is designed to aid in decision making, especially when the correct course of action is unclear, or it is...

1. Group Motivational Theories: Discuss which motivational theories work best with a group and why. Consider how AGT and SDT can enhance group performance. 2. Leadership and Motivation: Reflect on...

a. Create a Die class that you can use to instantiate objects that each hold a value from 1 through 6. The field that holds the value of the Die should be protected, which will allow a child class to...

Do you want to try your hand at producing a podcast? Businesses rely on a host of social media and communication technologies when reaching out to the public or internally to their workers. As you...

You own 250 shares of Dalton Resources preferred stock, which currently sells for $ 38.50 per share and pays annual dividends of $ 3.25 per share. a. What is your expected return? b. If you require...

TOPICS FOR DISCUSSION AND REVIEW 1. Compare and contrast the thermoregulatory strategies of marine birds and mammals. List two specific structural features that distinguish each of the following...

Digby has a leverage of 1 . 7 7 This means that: ( Assume leverage is calculated as Assets / Equity ) Select : 1 Save Answ $ 1 . 7 7 of assets is funded with $ 1 . 0 0 of equity and $ 0 . 7 7 of...

Transmission media are usually categorized as O a. guided or unguided O b. metallic or nonmetallic O c. determinate or indeterminate O d. fixed or unfixed

For what value of u do the homogenous equation have nontrivial solutions? Find these solutions (3 H)x1 x2 +x3 = 0 X1 - (1 u)x2 + x3 = 0 X1 x2 + (1 )x3 = 0

Data for Hermann Corporation are shown below: Per unit Percent of Sales Selling price $90 100% Variable expenses 63 70% Contribution margin $27 30% Fixed expenses are $30,000 per month and the...

Let v, = and y=-3. For what value(s) of h is y in the plane generated by v, and v?

Q#3 Find inverse of the block upper triangular matrix -1 -1 2 5 1 -3 8 A = 4 1 7 2

A weather forecaster states that there is a 78% chance of rain this weekend. Which word best describes the likelihood of it raining this weekend? impossible unlikely likely certain

For this assignment, you will write a descriptive essay, one in which you are expected to apply and demonstrate the principles of Description as discussed in this module. Instructions Brainstorm and...

Project should have: 1. one Java Project with the following files: 0 Printable.java (interface file) o Shape.java (abstract class) Circle.java inherits from Shape) Square.java (inherits from Shape) o...

M 5 A 2 Security Awareness in Action! Utilize the sites you identified within study exercise M 5 S 3 "Security Awareness" to complete this assignment. From the sites you selected, choose one that you...

Case: Delivering Bad News in an Orange Juice Factory Step 1 : Identify Issues The main issue in this case is the need to effectively communicate bad news givd e fully to the production department...

15 SLIDES REQUIRED PROJECT DESCRIPTION: Upper Coast Bank has branches all over the United States. Sophia Baker, the Vice President of Residential Lending at the Hartford, Connecticut branch, hired...

3 5 You want to use the Subtotal feature to display the average total deposit by number of bedrooms for each apartment complex. Use the Subtotal feature to insert subtotal rows by Apartment Complex...