Parikh et al. (2019) Biotech Letters describe an experiment in which the enzyme lipase B is...

 

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Parikh et al. (2019) Biotech Letters describe an experiment in which the enzyme lipase B is extracted from the yeast Candida antarctica - often a source of industrial lipase. The authors use the following esterification reaction to measure lipase activity: Capric acid + 1-propanol = propyl caprate + water H3C. HO но- -CH3 1L CH3 H3C a. The authors describe this reaction as having AG" = -2.35 kJ/mol and EAct = AG* = 37.79 kJ/mol. Draw a reaction coordinate plot for this reaction, showing how lipase affects the plot. b. At 30°C, the authors calculated Keg = 0.14. What is the ratio of capric acid to propyl caprate at equilibrium? Show your work! Parikh et al. (2019) Biotech Letters describe an experiment in which the enzyme lipase B is extracted from the yeast Candida antarctica - often a source of industrial lipase. The authors use the following esterification reaction to measure lipase activity: Capric acid + 1-propanol = propyl caprate + water H3C. HO но- -CH3 1L CH3 H3C a. The authors describe this reaction as having AG" = -2.35 kJ/mol and EAct = AG* = 37.79 kJ/mol. Draw a reaction coordinate plot for this reaction, showing how lipase affects the plot. b. At 30°C, the authors calculated Keg = 0.14. What is the ratio of capric acid to propyl caprate at equilibrium? Show your work!