Question: 6. Run multiple alignment programs using the attached glutathione peroxidases (gluperox). Start with Clustal Omega. use : >gi|285803075|gb|ADC35417.1| glutathione peroxidase [Pinctada fucata] MASGTKSQKIISPVKIENFHQFSAKTLQGETINFKQYEGKVILVENTASLUGVTEREMRAMNELVDKFDG RLVILGFPCNQFGKQENCTNKEILNCLKYVRPGDGYEPKFPLFEKRDVNGEKADPIFKFLRDRLPIPCDE STSLMTDPKSIIWSPVTRSDISWNFEKFLIDPTGKPHRRFSRYSQTKGLEKDVKTLIDEFNVV

6. Run multiple alignment programs using the attached glutathione peroxidases (gluperox). Start with Clustal Omega. use :

>gi|285803075|gb|ADC35417.1| glutathione peroxidase [Pinctada fucata] MASGTKSQKIISPVKIENFHQFSAKTLQGETINFKQYEGKVILVENTASLUGVTEREMRAMNELVDKFDG RLVILGFPCNQFGKQENCTNKEILNCLKYVRPGDGYEPKFPLFEKRDVNGEKADPIFKFLRDRLPIPCDE STSLMTDPKSIIWSPVTRSDISWNFEKFLIDPTGKPHRRFSRYSQTKGLEKDVKTLIDEFNVV >gi|264670920|gb|ACY72387.1| selenium-dependent glutathione peroxidase [Hyriopsis cumingii] MAVRIPLKPQNFFDVTAKTLKGQEVKLSKYKGKVILVENVASLUGTTVRDFTQMNELASKFEGKLVILGF PCNQFGHQENGNNDEILNSLKYVRPGNGFEPAFPIMEKCDVNGSNSHPLFQFLREKLQQPSDDPVSLMTH ANNIIWEPVTRTDISWNFEKFLIAPDGKPYKRYSRKFETKDIYNDIKDLIQQFGL

>gi|111572533|gb|ABH10623.1| selenium-dependent glutathione peroxidase [Unio tumidus] MAWEPSQAALALVAFCGVLYFAAGEEGRTKCTNTKNHTVHDFSFLNVYGNETIDLRYYRGEVLLVVNVAT YUGLTVQYHGSNALQGKYRNDSFRVLGVPCSQFHFQEPAFTSEELMNGLKYARPGHGFVPNFNLTQKTEI NGHKEHPLYTYIKSECPPARDRFVQPILYEPIYTSDVRWNFEKFLIGRDGHPVYRYASTIDPRTSQMLDA DIAVEIKKTLHGHGKDIVDIVG >gi|145559053|gb|ABP73388.1| selenium-dependent glutathione peroxidase [Dreissena polymorpha] MRGDGVLSALVSLAAIVGSTVGSEADNAIPPRQACRPPLGNQTIFDFSIRNVYDNATIDMSQFRGKLTLV VNVATYUGLVVQYHGLNALQTQHGADGFQVLGVPCNQFHYQEPAWNGVELTNGLKHMRPGGGFVPNFPLT LTTKVNGQDEHPMYKYLKSLCKSVYKKLYHPILYDPVYTEDVKWNYEKFLIGPDGIPIYRYAQTVDPATD AQFLADVKEELAKLKGHTHGPSSAPSAHVPVVG >gi|222875570|gb|ACM68948.1| selenium-dependent glutathione peroxidase [Macrobrachium rosenbergii] MSAIKSFYELSAKALSGETVSFSKYKGKVILVENTAALUGTTTRDFVQMNELKEKFGDALAVLAFPCNQF GHQENNTDEELLNTLKYVRPGNNFVPKMEVFGKVTVNGSEAHPIFKYLKERLPLPSDDSVSFMNDPQCII WKPVCRTDIAWNFEKFLIGKDGQPFKRYSKKYETKNIEEDINALLK

>gi|259124047|gb|ACV93251.1| glutathione peroxidase [Oreochromis niloticus] MAGHLKRFYDLTAKLLSGETLRFSALKDKVVLIENVASLUGTTTRDYTQVNDLHSRYSAEGLVILGVPCN QFGHQENCKNDEILRSLKYVRPGNGFEPKFQLLEKVDVNGKDAHPLFVYLKEKLPFPCDDALGLMNDPKY IIWSPVCRNDVSWNFEKFLVGPDGEPYKRYSRNFLTSDIEADIKQLLKRIK

>gi|32363469|gb|AAP80181.1| glutathione peroxidase 1 [Homo sapiens] MCAARLAAAAAAAQSVYAFSARPLAGGEPVSLGSLRGKVLLIENVASLUGTTVRDYTQMNELQRRLGPRG LVVLGFPCNQFGHQENAKNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSDDA TALMTDPKLITWSPVCRNDVAWNFEKFLVGPDGVPLRRYSRRFQTIDIEPDIEALLSQGPSCA >gi|169403976|ref|NP_001007282.2| glutathione peroxidase 1 [Danio rerio] MAGTMKKFYDLSAKLLSGDLLNFSSLKGKVVLIENVASLUGTTVRDYTQMNELHSRYADQGLVVLGAPCN QFGHQENCKNEEILQSLKYVRPGNGFEPKFQILEKLEVNGENAHPLFAFLKEKLPQPSDDPVSLMGDPKF IIWSPVCRNDISWNFEKFLIGPDGEPFKRYSRRFLTIDIDADIKELLKRTK >gi|84871986|ref|NP_032186.2| glutathione peroxidase 1 [Mus musculus] MCAARLSAAAQSTVYAFSARPLTGGEPVSLGSLRGKVLLIENVASLUGTTIRDYTEMNDLQKRLGPRGLV VLGFPCNQFGHQENGKNEEILNSLKYVRPGGGFEPNFTLFEKCEVNGEKAHPLFTFLRNALPTPSDDPTA LMTDPKYIIWSPVCRNDIAWNFEKFLVGPDGVPVRRYSRRFRTIDIEPDIETLLSQQSGNS >gi|32967607|ref|NP_002074.2| glutathione peroxidase 2 [Homo sapiens] MAFIAKSFYDLSAISLDGEKVDFNTFRGRAVLIENVASLUGTTTRDFTQLNELQCRFPRRLVVLGFPCNQ FGHQENCQNEEILNSLKYVRPGGGYQPTFTLVQKCEVNGQNEHPVFAYLKDKLPYPYDDPFSLMTDPKLI IWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKRLLKVAI

>gi|34783711|gb|AAH54848.2| Glutathione peroxidase 2 [Mus musculus] MAYIAKSFYDLSAIGLDGEKIDFNTFRGRAVLIENVASLUGTTTRDYNQLNELQCRFPRRLVVLGFPCNQ FGHQENCQNEEILNSLKYVRPGGGYQPTFSLTQKCDVNGQNEHPVFAYLKDKLPYPYDDPFSLMTDPKLI IWSPVRRSDVSWNFEKFLIGPEGEPFRRYSRSFQTINIEPDIKRLLKVAI

>gi|312600934|gb|ADQ92353.1| glutathione peroxidase [Mizuhopecten yessoensis] MATVVSVRSPVKLQNFFDFHAKTLDGETINFSKYKGKVILVMNVASLUDTTVRDYTQMNELVQKYGDKLV VLGFPCNQFGHQENTKNHEILTALKYLRPGDGFVPKFPIFEKLEVNGKNASEIFVFLRNHLPLPSDDPTS FMKSCSSIIWEPVTRTDLAWNFEKFLITPEGKPYKRFSRYYLTCNLQSEIKTLIEKFNVK

c. Compare the Clustal Omega output to the gluperox image. The researchers used ClustalW

to produce that figure. How close is the output to what is seen in the figure? Can you find any

major differences (other than order of sequences)?

d. The sequences all have one selenocysteine residue (u). At what amino acid

position is selenocysteine (use gi number 285803075 as your reference

sequence to determine the position)?

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