7. 116.5 ug of trypsin (MW 23.3 kDa) was mixed in a 1 ml solution with...

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7. 116.5 ug of trypsin (MW 23.3 kDa) was mixed in a 1 ml solution with an excess of a peptide substrate. The reaction is stopped after 10 seconds and the amount of cut peptide is measured to be 3.35 umole. The reaction is repeated with [S] = 0.012 M, and the amount of cut peptide is measured to be 1.175 umole in 10 seconds. a) What is the concentration of enzyme present? b) What is Vm ? c) What is KM ? d) What is kcat ? 7. 116.5 ug of trypsin (MW 23.3 kDa) was mixed in a 1 ml solution with an excess of a peptide substrate. The reaction is stopped after 10 seconds and the amount of cut peptide is measured to be 3.35 umole. The reaction is repeated with [S] = 0.012 M, and the amount of cut peptide is measured to be 1.175 umole in 10 seconds. a) What is the concentration of enzyme present? b) What is Vm ? c) What is KM ? d) What is kcat ?

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