Question: 7. [ 2 marks] In your experiment, you transformed E. coli bacteria with a naked pUC18 plasmid. If an insert had been ligated into the

7. [ 2 marks] In your experiment, you transformed E. coli bacteria with a naked pUC18 plasmid. If an insert had been ligated into the multiple cloning site (MCS) of the plasmid, would you have performed any aspect of the protocol differently? Why or why not? What would you expect to see on your plates after incubation? Explain

Step by Step Solution

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock blur-text-image

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Students Have Also Explored These Related Chemical Engineering Questions!

Name Lab M13 reverse priming site I Hind III KpnI Soci BamHI lads Bsex EcoRI ECORI EcoR V BseXt Not! Xho 1 Nisi! Xbo! Apa! TOPO Worksheet on interpreting transformation results 1) Last week in lab,...

2 Use the data provided in Sheet 3 named "Raw Data" to complete the missing items in the sheets 4 and 5 named "Monthly Return Template and "Solution Template" respectively. The use of calculators and...

Only equations, please!!!!!!!!!!! Only equations, please!!!!!!!!!!! Question 1 (40 marks) Refer to Figure 1. Write the Excel formula for each cell marked with ?? in column C. and label each formula...

Question 1 (40 marks) Refer to Figure 1. Write the Excel formula for each cell marked with "?? in column C. and label each formula clearly with cell reference position. TUV Investment Company manages...

\fc) 4+69+.... r : I mark S = 2 marks . . . . . . 5 . . 4 23. The sum of an infinite geometric series 18 5 and the common ratio is . Find the first term. 23. 2 nmrks 24. A hard rubber ball is dropped...

Please solve and show work IB style for 11 and 12 and 19 and 20. The answer key is attached but the numbering is skewed. However, there is no answer in the answer key for 11 e. I tried to correct the...

please i need the answer for my worksheet AutoSave . Off) H ASN_A7 - Compatibility Mode - Word O Search ezzat mal File Home Insert Draw Design Layout References Mailings Review View Help Share...

Please solve and show work IB style for 17 and 18. The answer key is attached but the numbering is skewed. I tried to correct the numbering. 36 (1 mark) Tower hears reply b 6 _1 (1 mark) Ship hears 4...

Please solve and show work IB style for 15 and 16. The answer key is attached but the numbering is skewed. I tried to correct the numbering. 36 (1 mark) Tower hears reply b 6 _1 (1 mark) Ship hears 4...

You have been hired by First Citizens Bank as a financial analyst. One of your first job assignments is to analyze the present financial condition of Bradley Stores, Incorporated. You are provided...

Forte Products is considering producing MP3 players and digital video recorders (DVRs). The products require different specialized machines, each costing $1.1 million. Each machine has a five-year...

The Closed Getaway Spawl has been so successful. Sold a bundle of Spawl services with the Merchandise Basket. When sold separately, the h service part of the bundle sells for $ 5 2 8 , and the...

What is the instruction for following RTL ? \ table [ [ \ table [ [ P C [ 1 0 H ]

Remove all filtering (it may be easier to temporarily remove the Store and State Hierarchy from the field list to answer this question). What is the overall Current Month Status for September 2016?

How does the ability to drill down into the state and store data give management critical information and help them identify issues that are occurring or opportunities that might be available?

What would you recommend that Dillards do regarding the past performance exhibited in the Day of the Week Dashboard?