8) A pesticide inhibits the activity of a particular enzyme A, which can therefore be used...
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8) A pesticide inhibits the activity of a particular enzyme A, which can therefore be used to assay for the presence of the pesticide in an unknown sample. a. In the laboratory, the initial rate data obtained are shown below. The rate, v, is measured in units of Ix10° mol/(L-min). Is the pesticide a competitive or noncompetitive inhibitor? Determine Kị, Vmax, and Km- [S] (mol/L) 3.3 х 104 5.0 x 104 6.7 x 10 1.65 x 10 2.21 x 103 v (no inhibitor) v(10*M inhibitor) 56 37 71 47 88 61 129 103 149 125 b. After 50 ml of the same enzyme solution in part (a) is mixed with 50 mL of 8x10 M substrate and 25 mL of sample, the initial rate observed is 18 umol/(min-L). Assuming that there are no other inhibitors or substrates present in the sample, what is the pesticide concentration in the unknown sample? 8) A pesticide inhibits the activity of a particular enzyme A, which can therefore be used to assay for the presence of the pesticide in an unknown sample. a. In the laboratory, the initial rate data obtained are shown below. The rate, v, is measured in units of Ix10° mol/(L-min). Is the pesticide a competitive or noncompetitive inhibitor? Determine Kị, Vmax, and Km- [S] (mol/L) 3.3 х 104 5.0 x 104 6.7 x 10 1.65 x 10 2.21 x 103 v (no inhibitor) v(10*M inhibitor) 56 37 71 47 88 61 129 103 149 125 b. After 50 ml of the same enzyme solution in part (a) is mixed with 50 mL of 8x10 M substrate and 25 mL of sample, the initial rate observed is 18 umol/(min-L). Assuming that there are no other inhibitors or substrates present in the sample, what is the pesticide concentration in the unknown sample?
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Related Book For
Fundamentals of biochemistry Life at the Molecular Level
ISBN: 978-0470547847
4th edition
Authors: Donald Voet, Judith G. Voet, Charlotte W. Pratt
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