Question: 9. You are performing experiments with dendritic cells isolated from TLR2+/- (heterozygous) or TIB2-/- (knockout) mice. You analyze a few different parameters after stimulating the
9. You are performing experiments with dendritic cells isolated from TLR2+/- (heterozygous) or TIB2-/- (knockout) mice. You analyze a few different parameters after stimulating the File Preview cento with lipopeptides (a ligand for TLR2/1). Draw the expected results for the following: a) western blot for IkBa on lysates of the stimulated dendritic cells TLR2+/- TLR2-/- b) surface expression of CD80 (a costimulatory molecule) on the dendritic cells, as measured by flow cytometry TLR2+/- TLR2-/- Cell counts Cell counts CD80 CD80
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