9:03 Done api.playposit.com 2 C Human genome project . It took 15 years - Sanger method 1988 1905 1999 2001 2003 AATCCG TAGG Today: more methods = cheaper Fluorescent Next gen sequencing - Illumina removable tags to 3rd gen sequencing- Single strand each dNTP CC What is the main difference between Sanger and Next gen sequencing? O That the terminators in Sanger sequencing are irreversible O That in Next gen sequencing there is fluoresence involved That Next gen sequencing uses faster polymerases That Sanger sequencing is the only that requires previous PCR SUBMIT 40:10 1:05:07