A biochemist wants to determine the effect of an inhibitor on a certain enzyme. The data...
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A biochemist wants to determine the effect of an inhibitor on a certain enzyme. The data are shown below: Vo (µM/min) (without inhibitor) Vo (uM/min) (with inhibitor) [Substrate] (HM) 10 13.2 7.9 20 20.9 12.2 40 28.4 17.2 80 36.6 22.1 120 40.2 24.2 o Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the absence of inhibitor. Vmax = + µM/min • Km = * µM o Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the presence of inhibitor. • Vmax = * µM/min Km = + µM o What is the type of inhibition involved? A biochemist wants to determine the effect of an inhibitor on a certain enzyme. The data are shown below: Vo (µM/min) (without inhibitor) Vo (uM/min) (with inhibitor) [Substrate] (HM) 10 13.2 7.9 20 20.9 12.2 40 28.4 17.2 80 36.6 22.1 120 40.2 24.2 o Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the absence of inhibitor. Vmax = + µM/min • Km = * µM o Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the presence of inhibitor. • Vmax = * µM/min Km = + µM o What is the type of inhibition involved?
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A Linear regression analysis without inhibitor V max 402 Mmin Answer K m of substrate conc... View the full answer
Related Book For
Introduction to Probability and Statistics
ISBN: 978-1133103752
14th edition
Authors: William Mendenhall, Robert Beaver, Barbara Beaver
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