A column has a length of 24.7 cm, and VM and V, of 1.37 mL and...

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A column has a length of 24.7 cm, and VM and V, of 1.37 mL and 0.165 mL, respectively. The unretained species passes through the column in 3.1 min. A and B are eluted at 13.3 and 14.1 min with the peak width (at base) of 1.07 and 1.16 min, respectively. Calculate the followings: (a) average number of theoretical plates in the column (b) plate height (c) retention factor of A (d) partition coefficient of A (e) column resolution for peaks A and B (f) number of plates to achieve resolution of 1.5 (g) length of column required for (f) (h) time required to elute A and B with the column of (g) A column has a length of 24.7 cm, and VM and V, of 1.37 mL and 0.165 mL, respectively. The unretained species passes through the column in 3.1 min. A and B are eluted at 13.3 and 14.1 min with the peak width (at base) of 1.07 and 1.16 min, respectively. Calculate the followings: (a) average number of theoretical plates in the column (b) plate height (c) retention factor of A (d) partition coefficient of A (e) column resolution for peaks A and B (f) number of plates to achieve resolution of 1.5 (g) length of column required for (f) (h) time required to elute A and B with the column of (g)

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To calculate the above parameters we can use the following formulas Number of theoretical plates N 1... View the full answer