A common buffer used to store DNA solutions is TE buffer Tris, $pH7\mathrm{.}6$ and $1$

mM EDTA$).$ The EDTA binds divalent cations and $\{$:$M{g}^{2+})$ that are cofactors for

DNase. How would you make $500mL$ of TE buffer starting with $0\mathrm{.}1M$ EDTA and $0\mathrm{.}05$

M Tris, $pH7\mathrm{.}4?$

Using the stock solutions made in questions $1$ and $2$ and solid NaCl, how would you

make $250mL$ GFP Extraction Buffer?

$50mM$ Tris$-HClpH7\mathrm{.}4$

$100$mMNaCl

$2$ mM EDTA, pH $8\mathrm{.}0$